Figure 4.

Diagram of the spontaneous deletions (D₆N₃₀ or N₆D₃₀) and prediction of protein structure of single-guide (sgRNA)/Cas9 targeted modifications of two MC1R-knockout (KO) rabbits. (A) “D₆” is designated as a spontaneous deletion (versus N₆ as nondeletion), with 6-bp deletion downstream of the ATG start codon. “D₃₀” is designated as spontaneous deletion (versus N₃₀ as nondeletion), with 30-bp deletion downstream of D₆. The CRISPR/Cas9 targeted modifications are illustrated separately in the upstream and downstream of spontaneous deletion diagram, which shows the forms of modifications, and predicted protein products of a premature stop codon of amino acids. The sgRNA sequences are marked in green, PAM sites are red, and the deletions are designated by green dashes. (B) Construction of MC1R E^D models according to five modifications and two WT types of BR. WT D₆, structure of MC1R E^D allele; WT D₃₀, structure of MC1R e allele; Y31 m1, editing type of −4 and −39 bp; Y31 m2, editing type of −278 or 302 bp; Y32 m1, editing type of −10 bp; Y32 m2, editing type of −28 bp; Y32 m3, editing type of −7 and −6 bp.