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Off-target detection in the MC1R-knockout (KO) rabbits. (A) PCR and T7E1 assays of the top eight potential off-target sites (POTS) for Sg1# and Sg2#, respectively. No fragment is found in T7E1 assays. (B) Chromatogram sequence analysis of 16 POTS for Sg1# and Sg2# using PCR products in the founders. No double curve was shown in any sequencing diagrams. The POTS are underlined in red.
