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. 2019 Sep 18;10:875. doi: 10.3389/fgene.2019.00875

Figure 6.

Figure 6

Phenotype identification and the expression of downstream genes in MC1R signal pathway of MC1R-KO rabbits. (A) H&E staining of the skin from the BR and KO rabbits. BR, Lianshan black rabbit; H&E, hematoxylin and eosin; KO, MC1R-knockout rabbits. Scale bar, 100 μm for 400×. (B) Integral optical density (IOD) analysis of histological sections by Image-Pro Plus software. (C) Relative expression of MITF, TYR, TYRP1, and DCT genes were determined by Real-time quantitative PCR (qRT-PCR). Bar graphs show mean values. Error bars represent standard error (SE). (D) The relation of MITF, TYR, TYRP1, and DCT in the melanogenesis pathway. The genes with a green frame are significantly downregulated in the KO skin compared with the BR skin. BR, Lianshan black rabbit; YR, Fujian yellow rabbit; KO, MC1R-KO rabbit. *0.01 < p < 0.05; **p < 0.01 (the diagram of melanogenesis pathway was simplified from the KEGG pathway database, 04916, 10/23/15, Kanchisa Laboratories).