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Summary of the MC1R-knockout (KO) rabbits generated by CRISPR/Cas9.
| sgRNA | gRNA/Cas9 mRNA (ng/μl) | Embryos injected | Embryos transferred | Pregnancy | Pups obtained (% transferred) | MC1R-KO pups (% pups) | Pups with color change | |
|---|---|---|---|---|---|---|---|---|
| 1# | Sg1 + Sg2 | 40/100 | 13 | 13 | Yes | 7 (53.8%) | 0 | 0 |
| 2# | Sg1 + Sg2 | 40/100 | 17 | 16 | No | – | – | – |
| 3# | Sg1 + Sg2 | 40/100 | 14 | 14 | Yes | 2 (14.3%) | 2 (100%) | 2 |
| 4# | Sg1 + Sg2 | 40/100 | 14 | 14 | No | – | – | – |
