Table 1.

Study sample description.

Patient	Age range	Diagnosis according to clinical features	Expressed transcription factor	Extrasellar extension	Primary PA or relapse	Medical therapy before sampling	sDNA Sanger sequencing results	cfDNA analysis method	Number of variants in sDNA from WES	Transcripts obtained for NGS analysis
HA065	6−69	NFPA	SF1	Yes	Primary	No	No variant	NGS	272	3
HA066	50–59	NFPA	TPIT	Yes	Primary	No	No variant	NGS	671	2
HA067	70–79	NFPA	SF1	Yes	Relapse	No	No variant	NGS	6	5
HA068	60–69	NFPA	nd	Yes	Primary	No	No variant	NGS	1	0
HA069	60–69	NFPA	nd	Yes	Primary	No	No variant	NGS and cast-PCR	4	0
HA070	70–79	NFPA	nd	Yes	Relapse	No	No variant	NGS and cast-PCR	12	4
HA073	50–59	NFPA	nd	Yes	Primary	No	No variant	NGS and cast-PCR	10	2
HA074	40–49	GHPA	PIT1	No	Primary	Yes	GNAS c.601C>T	cast-PCR	3	–
HA090	50-59	GHPA	PIT1	No	Primary	No	GNAS c.601C>T	NGS & cast-PCR	2	1
HA091	40-49	GHPA	PIT1	Yes	Primary	No	GNAS c.601C>T	cast-PCR	5	–

sDNA, somatic DNA; cfDNA, circulating free DNA; WES, whole-exome sequencing; IHC(P), immunohistochemical analysis done on formalin-fixed paraffin-embedded samples; GHPA, somatotropinoma; NFPA, non-functional pituitary adenoma; nd, no data, not performed.