Table 1.
Primers used in this study.
| Primers | Purpose | Sequence (5′-3′) |
|---|---|---|
| DuSTING-ORF-24 U | To obtain sequence | GGTGTCCTGGCCCTGGTCGCTCCG |
| DuSTING-ORF+30 L | GCGGGAGGCTCCTGCTGCAGGACG | |
| EcoR I DuSTING U | Cloning | TAGTCCAGTGTGGTGUGAATTCATGTCTCAGGAACCGCAGCACCG |
| Xho I DuSTING L | GTCGTCCTTGTAGTCUCTCGAGGGGGTGGTCGCTCCGCAGGG | |
| qDuSTING | qRT-PCR | ACAAGCACAGCCTCTACGCAATC |
| qDuSTING L | CGCAATGAGCCTGTAGGTTCC | |
| qIFN-β U | CCTCAACCAGATCCAGCATT | |
| qIFN-β L | GGATGAGGCTGTGAGAGGAG | |
| qIL-1β U | TGGGCATCAAGGGCTACAAG | |
| qIL-1β L | GCTGTCGATGTCCCTCATGAC | |
| qIL-8 U | GAGCCTGGTAAGGATGGGAAA | |
| qIL-8 L | CTGCGTCAGCTTCACATCTTG | |
| qIRF1 U | AGCACCAACGACATCTACCAG | |
| qIRF1 L | GAACTCCAACTCTGCCGAAG | |
| qIRF7 U | GCCTGAAGAAGTGCAAGGTC | |
| qIRF7 L | CTCTGTGCAAAACACCCTGA | |
| DuSTING 1 U | Construct truncated forms of DuSTING | CTCGAGGACTACAAG GACGACGATG |
| DuSTING 1-180 L | CTTGTAGTCCTCGAG CTCCTTTATGCGTGGCAGAA | |
| DuSTING 1-250 L | CTTGTAGTCCTCGAG GATTGCGTAGAGGCTGTGCT | |
| DuSTING 1-350 L | CTTGTAGTCCTCGAG GCTCCCCTCGTACACCGTGA | |
| DuSTING 1-368 L | CTTGTAGTCCTCGAG GATCTGGAGGCTGAGATCTG | |
| DuSTING 9-382 U | AGTGTGGTGGAATTC ATG AGCAGCCCCGCTGCCCTGC | |
| DuSTING 44-382 U | AGTGTGGTGGAATTC ATG GAGCCCCTGTCCCCCGCTGC | |
| DuSTING 85-382 U | AGTGTGGTGGAATTC ATG GGCAGCTTCTGGAGGGCCCT | |
| DuSTING 117-382 U | AGTGTGGTGGAATTC ATG GGAGAGAGGCTCAGCCCCCA | |
| DuSTING 149-382 U | AGTGTGGTGGAATTC ATG GAGATGACCGAGAGGTCCCA | |
| DuSTING 181-382 U | AGTGTGGTGGAATTC ATG TGTATGGAGGAAATCAGCAG | |
| DuSTING 382 L | GAATTCCACCACACT GGACTAGTGG |
Homology arm sequences are highlighted in bold and italics. The restriction enzyme sites are underlined.