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. 2019 Sep 18;13:989. doi: 10.3389/fnins.2019.00989

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Copyright © 2019 You, Wu, Zhou, Zhang, Yuan, Wen, Xu, Cui, Tang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of neuronal uptake between Fe₃O₄/ATX and Fe₃O₄/ATX/Transferrin. (A) Typical confocal images showed primary cultured neurons in Fe₃O₄/ATX group and Fe₃O₄/ATX/Transferrin group at 6 h after incubation. (B) The intensity in relative fluorescence of Nanoparticles: Neurons. Images were standardized to the same setting. The green, the blue, and the red indicates fluorescence of MAP2, DAPI, and AF647-labeled NPs, respectively. Values are expressed as the means ± SEM. ^∗p < 0.05 vs. Fe₃O₄/ATX group; ns p > 0.05 vs. Fe₃O₄/ATX group.