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. 2019 Sep 18;13:989. doi: 10.3389/fnins.2019.00989

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Copyright © 2019 You, Wu, Zhou, Zhang, Yuan, Wen, Xu, Cui, Tang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison between ATX and Fe₃O₄/ATX/Transferrin treatment on neuronal damage in cultured neurons exposed to OxyHb. (A) Representative Western blots showed the expression of Bcl-2, Bax, and cleaved caspase-3 (CC3) at 12 h after OxyHb exposure in different groups. (B,C) Relative intensity analysis of Bcl-2, Bax, cleaved caspase-3 (CC3) and β-actin among all experimental groups, respectively. (D) Apoptosis detection results after OxyHb exposure were detected by flow cytometry. (E) The ratio of apoptotic cells in each group was measured. Values are expressed as the means ± SEM. ^#p < 0.05 vs. Control group; ^∗p < 0.05 vs. SAH group; ^∗∗p < 0.01 vs. SAH group.