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. 2019 Sep 18;7:638. doi: 10.3389/fchem.2019.00638

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Copyright © 2019 Dravid, Raos, Aqrawe, Parittotokkaporn, O'Carroll and Svirskis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rheological behavior of 0.2% collagen (Col), 1% agarose (AG) and 0.03% collagen+1% agarose co-gel (AG-Col) determined through parallel-plate rheometry. (A) Storage (shaded symbols) and loss (open symbols) moduli represent the elastic and viscous properties of the material. For all hydrogels the storage modulus exceeds the loss, indicating that the hydrogel is present in its gel state. (B) The complex modulus is used to represent the stiffness of the hydrogels. A statistically significant difference is observed between all groups, yet the stiffness for all remains within appropriate range for neuronal culture. Each data point represents the mean (n = 3) ± SD. (**p < 0.01 and ***p < 0.001).