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. 2019 Sep 18;7:638. doi: 10.3389/fchem.2019.00638

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Copyright © 2019 Dravid, Raos, Aqrawe, Parittotokkaporn, O'Carroll and Svirskis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The biocompatibility of the gradient-generator and hydrogels used was investigated using a LIVE/DEAD® viability/cytotoxicity assay on SH-SY5Y neurons after 96 h in culture. (A) LIVE/DEAD® assay fluorescence and brightfield images visualized using a 10× objective. (B) The viability of the neurons was calculated by determining the number of live cells (dual labeled with Hoechst nuclear stain and calcein) as a percentage of the total number of cells. Cells grown on polystyrene and glass were used as the positive control. Each bar represents the mean (two separate regions, three independent replicates n = 6) ± SD. (C) Morphology of neurons cultured on Matrigel-coated polystyrene (20× objective). (D) Morphology of neurons cultured on the Matrigel-coated gradient-generator overlaid with thin agarose hydrogel (20× objective).