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CyaA dedifferentiates human alveolar macrophages. (A) Scheme of the experiment. Alveolar macrophages obtained from human bronchoalveolar lavage (BAL) fluid samples were treated with 4 ng/ml (22.5 pM) or 8 ng/ml (45 pM) of CyaA for 5 days in the presence of M-CSF. (B and C) Macrophage markers (B) and CD14 cell surface expression (C) were analyzed by flow cytometry. n = 5 biological replicates; *, P < 0.05; **, P < 0.005; ***, P < 0.0005.
