FIG 4.

HEAL-FUS complex recruits histone acetyltransferase p300 to modulate histone modification and P-TEFb enrichment at the HIV promoter. HIV-infected control or HEAL knockdown MT4 cells were prepared for CHIP analyses—p300 (A), H3K27ac (B), and cyclin T1 (C)—as described in the legend to Fig. 3F. Results of RT-qPCR of the HIV promoter regions enriched for p300, H3K27ac, and cyclin T1 are shown in panels A, B, and C, respectively. Mean ± SD of n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.