Figure 1.

Analysis of RL2:pEGFP complex formation. (A) Gel retardation assays of the RL2:pEGFP complex. N/P ratios of RL2/pDNA charge have varied from 0.5 to 5 in reaction mixtures; C- control (free pDNA). Particle size (B) and ζ potential (C) of RL2:pEGFP complexes were analyzed for N/P ratios 5, 7, and 9. Reactions were performed in MES buffer for 5 min at 37°C. Particle size was estimated by the DLS technique. The data are representative of three independent repeats and are shown as the mean ± SD.