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. 2019 Sep 18;10:1043. doi: 10.3389/fphar.2019.01043

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Copyright © 2019 Chinak, Golubitskaya, Pyshnaya, Stepanov, Zhuravlev, Richter and Koval

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transfection of cancer cells by RL2:pEGFP at charge ratios from 5 to 9. (A) Cells were incubated with LF—Lipofectamine 3000 and pEGFP, free pEGFP or RL2-pEGFP complexes with various N/P ratios and 24 h after EGFP was analyzed by fluorescent microscopy. (B) Cells were pre-treated with anti-RL2 antibodies (ab) for 3 h and next transected with a complex RL2:pEGFP. (C) Electrophoretic analysis of the products of RT-PCR reaction with EGFP-specific primers. Free pUC19 and pEGFP were used as matrixes in PCR reaction for the negative and positive controls, respectively. Representative pictures of three independent experiments.