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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Radiat Res. 2019 Jul 11;192(3):267–281. doi: 10.1667/RR15379.1

FIG. 5.

FIG. 5.

RT-PCR detection of radiation-induced genes from Serpinb3a−/− and Serpinb3a+/+ Balb/c background strain bone marrow stromal cells. RNA was isolated from Serpinb3a−/− and Balb/c bone marrow stromal cells at 0 and 24 h after 10 Gy irradiation. Quantitative-PCR-RNA transcripts of stress response genes and inflammatory cytokines were performed with bone marrow stromal cell lines. RT-PCR in Serpinb3a−/− cells demonstrated increased radiation-induced mRNA for (panel A) MnSOD and (panel D) p21, and decreased expression of (panel B) p53 and (panel C) TGF-β. (Data with other radiation-induced RNA species are shown in Supplementary Fig. S5; http://dx.doi.org/10.1667/RR15379.1.S1). (n = 4 per data point). *P < 0.05 compared to Serpinb3a+/+ Balb/c background, 0 Gy; #P < 0.05 compared to Serpinb3a+/+ Balb/c background, 10 Gy. Results were standardized to Serpinb3a+/+ Balb/c background.