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. 2019 Sep 18;10:1150. doi: 10.3389/fpls.2019.01150

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Copyright © 2019 Aliaga-Franco, Zhang, Presa, Srivastava, Granell, Alabadí, Sadanandom, Blázquez and Minguet

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Position of each CRISPR target in selected genes At5g55250 (Arabidopsis thaliana), Solyc07g64990 and Solyc12g14500 (Solanum lycopersicon), and Os04g56950 (Oryza sativa). (B) Description of transcriptional units (arrows) assembled in the vectors generated for plant transformation using the modular system GoldenBraid. Specific promoters were selected for expression of Cas9 protein in each species, pAtUBQ10 in Arabidopsis thaliana, p35S for Solanum Lycopersicum, and pZmUBQ for Oryza sativa. Adequate promoter for expression of sgRNA was selected: pAtU6-26 for dicotyledonous species (Solanum and Arabidopsis) and pOsU3 for monocotyledonous species (rice).