Figure 3.

TLR2 mediates GAS-induced Txnip degradation. Western blotting showed Txnip and Trx expression in (A) RAW264.7 cells infected with GAS strain A20 (serotype M1) or S. aureus, (B) cells stimulated with inactivated GAS (HK-GAS) for 2 h, and (C) LTA-treated RAW264.7 cells. (D) In the presence or absence of MG132, cells were treated with HK-GAS or LTA for 2 h followed by the detection of Txnip expression. (E) Txnip expression was measured in wild-type (WT) or Tlr2^−/− BMDMs infected with GAS (MOI 10 and 50) or stimulated with HK-GAS or LTA for the indicated times. GAPDH and β-actin were used as internal controls. The expression ratios of Txnip and Trx to internal controls are shown. Protein molecular weights (MW) are indicated in kilodaltons. Western blot results represent at least two independent experiments.