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. 2019 Sep 18;10:2147. doi: 10.3389/fimmu.2019.02147

Figure 5.

Figure 5

Txnip silencing facilitates TLR2-mediated iNOS/NO, TNF-α, and IL-6 production. (A) Western blotting showed the expression levels of Txnip and GAPDH in RAW264.7 cells transfected with control siRNA or Txnip siRNA (5 nM) for 24 h. (B) iNOS expression and (C) NO production were detected in control siRNA- or Txnip siRNA-expressing cells stimulated with HK-GAS (MOI 10), LTA (5 μg/ml), and PGN (5 μg/ml) for 24 h. GAPDH was used as an internal control. The expression ratios of Txnip and iNOS to internal controls are shown. Nitrite concentrations are shown as the means ± SD of triplicate cultures. **p < 0.01; ***p < 0.001. The levels of (D) TNF-α and (E) IL-6 were measured in control siRNA- or Txnip siRNA-expressing cells that were treated with HK-GAS or PGN for 24 h and are shown as the means ± SD of triplicate cultures. ***p < 0.001 compared with control siRNA-expressed cells. Protein molecular weights (MW) are indicated in kilodaltons. Western blot results represent at least two independent experiments.