Figure 7.

A_2A-AR protects against cardiac release of AST (aspartate transaminase). Effects of A_2A-AR after ischemia and reperfusion on the activity of AST are presented. Isolated perfused hearts were prepared from WT and A_2A-TG and subjected to global ischemia for 20 min, followed by reperfusion. Venous effluates (1 ml) were collected before ischemia (Ctr) and after 40 min of reperfusion. In these effluates, AST enzyme activity was assessed (as % of Ctr). The protective effects of the A_2A-AR were abolished by the A_2A-AR antagonist ZM 241385 (1 µM). ^★p < 0.05 vs. WT, + p < 0.05 vs. Ctr, ^#p < 0.05 vs. without ZM 241385.