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. 2019 Sep 18;7:198. doi: 10.3389/fcell.2019.00198

FIGURE 1.

FIGURE 1

ER stress leads to the release of intracellular proteins. (A) Schematic of the treatment and purification protocol. (B) Image of the 40,000 xg pellets from a representative experiment. Pellet margins are highlighted. (C) Coomassie staining of the 1000 xg and 40,000 xg fractions. Fractions were run on a 4–20% SDS-PAGE gradient gel. The broad band running between the 50 and 70 kDa markers is bovine serum albumin from the media. Unique proteins identified by mass spectrometry in ER stressed cells are indicated by gene name.