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. 2019 Sep 18;10:1042. doi: 10.3389/fphar.2019.01042

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Copyright © 2019 Liu, Zhu, Wu, Shen, Lin, Wu, Liu, Tang, Zhou, Sun and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NAT-F induced DNA damage in NSCLC cells. (A) The DNA damage regulatory proteins in PC9 and H1299 cells were detected by Western blotting. In PC9 cells, samples of p-Chk1, Chk1, and Cdc25A were from the same gels; samples of GAPDH and γ-H2AX were from the same gels. In H1299 cells, samples of p-Chk1, Chk1, Cdc25A, and GAPDH were from the same gels; samples of γ-H2AX were from the same gels. GAPDH was used as internal control. Western blot assay was carried out at least 3 times, and data represented mean ± SD. Significant differences were indicated by ***p < 0.001 versus the control. (B) Immunoﬂuorescence images (630× magnification) of γ-H2AX in PC9 and H1299 cells treated with or without NAT-F(1 μM) treatments for 36 h, respectively. The red ﬂuorescence indicated γ-H2AX foci and the blue ﬂuorescence indicated cell nuclei, respectively. Bar: 50 μm. (C) Immunostaining of 8-OHdG in PC9 and H1299 cells with or without NAT-F(1 μM) treatments for 36 h. Representative images (630×magnification) of DAPI (blue color), 8-OHdG staining (green color), and merged were shown for each group of cells. Bar: 50 μm.