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. 2019 Sep 18;10:1042. doi: 10.3389/fphar.2019.01042

Figure 5.

Figure 5

Effect of NAT-F on MMP and apoptosis-related proteins in NSCLC cells in vitro. (A) Treated cells were stained with TMRM solution and then observed under a fluorescence microscope. Bar: 50 μm. (B) Histograms of the TMRM-stained cells by flow cytometry and bar graph of the MMP changes in NAT-F–treated PC9 and H1299 cells. (C) Western blot analysis of Bcl-2 family members (Bax, Bcl-2, Bcl-xL, and Mcl-1), cytochrome c, cleaved-caspase-9/-3 and cleaved-PARP expressions in PC9 and H1299 cells, and expression level were quantified. In PC9 cells, samples of cleaved-PARP, GAPDH, Bax, Bcl-xL, and Mcl-1 were from the same gels; samples of Bcl-2, cleaved caspase-9/-3, cytochrome c (cytoplasm) were from the same gels. In H1299 cells, the gels of these protein samples were similar to that of PC9 cells. GAPDH was used as internal control. Western blot assay was carried out at least three times, and data represented mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, significant difference between NAT-F-treated groups and the control.