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. 2019 Sep 18;10:862. doi: 10.3389/fgene.2019.00862

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Copyright © 2019 Elli, de Sanctis, Bergallo, Maffini, Pirelli, Galliano, Bordogna, Arosio and Mantovani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative 2-D scatterplots obtained from the raw imaging data analysis of rare mutation detection analysis using the QuantStudio™ 3D AnalysisSuite™ Software (upper panel: GNAS_27895_mu assay; lower panel: GNAS_27877_mu assay). Blue dots represent the FAM™ reporter dye signal that allows to identify and count mutant alleles present in the nanoscale-sized reaction wells of the chip. Yellow dots are the assay control signal and represent the absence of FAM™ signal in part of the nanoscale-sized reaction wells of the chip.