Table 2.

Gene ontology (GO) term annotation of genes preferentially expressed in (i) EEF stages and (ii) asexual EF stages

Biological process ID	Process description	Annotated	Observed	P value
(i) Preferentially expressed in exo-erythrocytic stage (24 h, 48 h, 54 h and 60 h)
GO:0006633	Fatty acid biosynthetic process	9	6	4e−06
GO:0044409	Entry into host	33	7	0.0036
GO:0006272	Leading strand elongation	2	2	0.0040
GO:0033014	Tetrapyrrole biosynthetic process	9	4	0.0042
GO:0006260	DNA replication	39	10	0.0110
GO:0055114	Oxidation–reduction process	83	12	0.0215
GO:0006270	DNA replication initiation	4	2	0.0219
GO:0006334	Nucleosome assembly	4	2	0.0219
(ii) Preferentially expressed in erythrocytic stage (ring 4 h, trophozoite 16 h)
GO:0006928	Movement of cell or subcellular component	34	6	0.00036
GO:0044053	Translocation of peptides or proteins into host cell cytoplasm	3	2	0.00256
GO:0040011	Locomotion	22	5	0.00644
GO:0035891	Exit from host cell	8	2	0.02169
GO:0009405	Pathogenesis	8	2	0.02169
GO:0030833	Regulation of actin filament polymerization	1	1	0.02978
GO:0006166	Purine ribonucleoside salvage	1	1	0.02978
GO:0019510	S-Adenosylhomocysteine catabolic process	1	1	0.02978
GO:0010323	Negative regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway	1	1	0.02978
GO:0020035	Cytoadherence to microvasculature, mediated by symbiont protein	1	1	0.02978
GO:0007050	Cell cycle arrest	1	1	0.02978

The number of annotated genes (number genes per GO term present in the entire RNA-seq study) and observed genes (number of genes per GO term preferentially expressed in…) per Biological Process are listed. Only GO terms with P-values < 0.05 are shown