Table 3.

Gene ontology (GO) term annotation of genes preferentially expressed in (i) EEF merozoites and (ii) EF merozoites

Biological process ID	Process description	Annotated	Observed	P value
(i) Preferentially expressed in exo-erythrocytic stage merozoites (DCs/merosomes)
GO:0010467	Gene expression	462	181	3.0e−22
GO:0042254	Ribosome biogenesis	63	47	4.6e−20
GO:0043604	Amide biosynthetic process	219	98	1.1e−17
GO:0032774	RNA biosynthetic process	103	35	0.00017
GO:0017183	Peptidyl-diphthamide biosynthetic process from peptidyl-histidine	5	4	0.00700
GO:0000398	mRNA splicing, via spliceosome	43	15	0.01718
GO:0008295	Spermidine biosynthetic process	2	2	0.04105
GO:0008612	Peptidyl-lysine modification to peptidyl-hypusine	2	2	0.04105
(ii) Preferentially expressed in erythrocytic stage merozoites (22 h schizonts)
GO:0007264	Small gtpase mediated signal transduction	26	14	0.0014
GO:0006260	DNA replication	39	20	0.0049
GO:0045184	Establishment of protein localization	93	34	0.0060
GO:0007017	Microtubule-based process	35	15	0.0075
GO:0000041	Transition metal ion transport	7	6	0.0151
GO:0006465	Signal peptide processing	5	4	0.0153
GO:0006848	Pyruvate transport	3	3	0.0154
GO:0006497	Protein lipidation	21	10	0.0196
GO:0006511	Ubiquitin-dependent protein catabolic process	38	16	0.0248
GO:0043248	Proteasome assembly	8	5	0.0267
GO:0006310	DNA recombination	9	6	0.0366
GO:0006468	Protein phosphorylation	82	28	0.0437

The number of annotated genes (number genes per GO term present in the entire RNA-seq study) and observed genes (number of genes per GO term preferentially expressed in…) per Biological Process are listed. Only GO terms with P-values < 0.05 are shown