Fig. 3.

Detection of hEPO protein in mouse blood after hEPO mRNA delivery via EVs Mice were intravenously injected with 100 μL of mc3-EVs containing 1.5 µg of hEPO mRNA (per mouse). The concentrations of hEPO protein in murine plasma were determined by Gyros immunoassay for hEPO at 0 (untreated), 2, 5, and 24 h after EV injection. The hEPO protein was detected in mouse blood at 2 h after EV injection. N = 8 independent animals at each time point except for 2 h (n = 4) are presented. The plasma hEPO protein from mc3-EV delivery was compared between 2 and 5 h by unpaired two-tailed Student’s t-test, although the difference was not statistically significant (ns = not significant). Source data are provided as a Source Data file