Fig. 4.

Quantification of hEPO mRNA and hEPO protein in mouse organs. Mice were intravenously injected with 100 μL of mc3-EVs containing 1.5 µg of hEPO-mRNA (per mouse). At 5, 24, and 96 h after EV injection, the levels of hEPO mRNA and hEPO protein were determined in eight organs by qPCR and ELISA, respectively. a, b Levels of hEPO mRNA and protein in the heart, c, d lung, e, f liver, g, h spleen, i, j kidney, k, l thymus, m, n pancreas, and o, p brain are shown. The highest amount of hEPO protein was detected in the liver, whereas the highest amount of hEPO mRNA was detected in the spleen. Data are presented as the mean (bars) and standard deviation (SD) n = 4 independent animals at each time point. EVs and untreated groups were compared at each time point using the unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01, and ***p < 0.001. Source data are provided as a Source Data file