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. 2019 Sep 24;9:13797. doi: 10.1038/s41598-019-50310-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global changes in gene expression at 24 hours. Euler plots of the total number of significantly differentially expressed genes (DEGs, FDR-adjusted p-value < 0.01) associated with each test group 24 hours after the second dose. (A) Overlap between DEGs associated with 4CMenB, and each of its alum-adsorbed components. (B) Overlap between 4CMenB, each of the alum-adsorbed deoxycholate and native outer membrane vesicles (dOMVs and nOMVs), and alum-adsorbed lipopolysaccharide (LPS) from Escherichia coli.