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. 2019 Sep 24;9:13797. doi: 10.1038/s41598-019-50310-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of cytokines in mouse sera. Log₂ median fluorescence intensity (MFI) values for each of cytokine detected by a six-plex cytokine bead assay 24 hours after immunisation with (A) 4CMenB, one of its constituent components, or one of several comparator antigens, or (B) 4CMenB + routine, routine only, or PBS control immunisations. Kruskal-Wallis significance values are presented in the top left corner of each panel. Significance of group medians relative to naïve or PBS controls were calculated using Mann-Whitney rank-sum tests. *< 0.05.