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. 2019 Sep 24;10(10):709. doi: 10.1038/s41419-019-1872-y

Fig. 2. Treatment with CP-dn-ATF5 causes a dose- and time-dependent depletion of survivin protein in multiple cancer cell lines prior to loss of cell viability.

Fig. 2

a, b CP-dn-ATF5 depletes survivin in T98G, U87 and LN229 glioblastoma cell lines at 24 h after treatment. a Representative western blots. b Data are from three independent experiments. c CP-dn-ATF5 depletes survivin in T98G, U87 and LN229 glioblastoma cell lines at 48 and 72 h after treatment. Normalized survivin to ACTIN ratios are shown for each lane. d Treatment with CP-dn-ATF5 causes a rapid depletion of survivin protein in T98G cultures. Normalized survivin to ACTIN ratios are shown for each lane. e Cell death caused by CP-dn-ATF5 (100 µM) in T98G cultures occurs after survivin depletion. Relative numbers of cells per culture were determined at the indicated times. Three replicate cultures were evaluated at each time point