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. 2019 Sep 24;9:13753. doi: 10.1038/s41598-019-50276-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of CLDN1 and CLDN11 in human normal lung and SCC cells. (A) The expression levels of CLDN1 and CLDN11 mRNAs in human lung SCC tissue are shown as a percentage of the values in normal lung tissues. (B) The expression levels of CLDN1 and CLDN11 mRNAs in RERF-LC-AI cells, derived from human lung SCC are shown as a percentage of the values in normal lung tissues. (C) Immunofluorescence staining with anti-CLDN1, anti-CLDN11 (red), and anti-ZO-1 (green) antibodies was performed. The right-hand images show merged pictures with DAPI (blue). Scale bar represents 10 µm. n = 3–4. **P < 0.01 and *P < 0.05 compared with normal. NS, P > 0.05.