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. 2019 Sep 24;9:13753. doi: 10.1038/s41598-019-50276-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of CA and knockdown of CLDN on tight junction permeability. RERF-LC-AI cells were plated on Transwell inserts. (A) TER was analyzed on days 0, 2, 5, 7, and 10 using a volt ohmmeter. (B,C) After culturing for 4 days, the cells were incubated in the presence or absence of 10 mM CA for 2 h. TER was analyzed using a volt ohmmeter. Transepithelial lucifer yellow and DXR fluxes were analyzed using a fluorescence spectrometry. (D,E) The cells were transfected with siRNAs for negative control, CLDN1, or CLDN11. TER and transepithelial flux were analyzed. n = 3–4. **P < 0.01, *P < 0.05, and NS, P > 0.05 compared with the negative control siRNA.