Skip to main content
. 2019 Sep 24;9:13753. doi: 10.1038/s41598-019-50276-z

Figure 7.

Figure 7

Association of chrysin with Akt. (A) Reaction scheme for the synthesis of biotinylated chrysin. (B) Cell lysates were incubated with or without biotinylated-chrysin in the presence of avidin agarose. Precipitated proteins were subjected to western blotting. The protein levels of precipitated Akt and PDK1 are shown as a percentage of values in the input. The full-length blot images are shown in Supplementary Fig. S4. (C) The QCM frequency was measured every 1 s. Then, 10 ng/mL biotinylated-chrysin was applied at the time period indicated by the hatched box. (D) Cell lysates prepared from the cells treated with or without 10 μM chrysin were incubated with protein G sepharose beads and anti-PDK1 antibody. Precipitated proteins were subjected to western blotting. IgG indicates the band of heavy chain of immunoglobulin G. The protein levels of precipitated Akt and PDK1 are shown as a percentage of values in the input. The full-length blot images are shown in Supplementary Fig. S4. n = 3–4. **P < 0.01 and NS, P > 0.05 compared without biotinylated-chrysin or chrysin.