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. 2019 Sep 24;9:13778. doi: 10.1038/s41598-019-50360-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measurement of gastric fluid pepsin concentration. Pepsin concentration was measured in gastric fluid of patients on and off PPI therapy. Gastric fluid from patients on therapy had significantly higher concentrations of pepsin than patients off therapy (p = 0.03). Both on and off PPI therapy groups represent the mean +/− SEM of pepsin measured in the gastric fluid derived from 12 patients/group (n = 12). Pepsin concentrations were log transformed and differences were considered significant between the two patient groups at p ≤ 0.05 and noted by the symbol (*). The p values were determined using an unpaired two-tailed student’s T test with equal variance. Supplementary Fig. S2 depicts pepsin concentration within the gastric fluid of each individual patient sample, with numerical values of pepsin concentration listed in Supplementary Table S1.