Fig. 2. CBS deficiency increased the ratio of granulocyte/erythroid cells, iron, Ft-L, and Ft-H contents, and reduced expression of ALAS2, FECH, and FLVCR mRNAs in the bone marrow.

a Bone marrow smear (granulocyte/erythroid cells); b Prussian blue staining (iron) in the bone marrow; c–j Immunofluorescence analysis of the expression of Ft-L (c and e) and Ft-H (d and f) proteins in the bone marrow; and G-K: Real-time PCR analysis of the expression of Ft-L (g), Ft-H (h), ALAS2 (i), FECH (j) and FLVCR (K) mRNAs in the bone marrow of CBS^+/+ (a), CBS^+/− (b), and CBS^−/− (c) mice. Data are means ± SEM (n = 6). *p < 0.05, **p < 0.01 vs. CBS^+/+ or CBS^+/− mice