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. 2019 Sep 24;9:13752. doi: 10.1038/s41598-019-50287-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional validation of AGO2 knockout cells. (A) RNAi reporter assay in HeLa WT control and two AGO2 KO cell clones. Cells were transfected with expression plasmids encoding firefly luciferase (Fluc), Renilla luciferase (Rluc), either Fluc specific shRNA (shLuc) or a non-targeting control shRNA (shCtrl), along with expression plasmids encoding wildtype AGO2 or slicer deficient AGO2 D597A where indicated. Fluc counts were normalized to Rluc counts and expressed as a percentage of the non-targeting control shRNA. Data are presented as means and SD of three biological replicates. Data are from one experiment representative of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test. (B) Scatter plot representing miRNA levels in WT and AGO2 KO2 HeLa cells. Reads are normalized to library size. Averaged data from EMCV infected HeLa cells at 8 and 10 hpi are presented (plots for the individual time points in Supplementary Fig. 2). Selected miRNAs are indicated in red.