FIG. 7.

Representative H&E staining of explanted scaffolds (n = 6 for each group at each time point, except for the +Enteroid group at 12 weeks (n = 5) and the −Enteroid group at 2 weeks (n = 5). (A) Tile scan views of an unseeded scaffold after 12 weeks of implantation (top) and seeded scaffold (bottom) at 200 × magnification. The scaffold is surrounded by tissue with it covering the villi in this case. (B) A magnified view of the black box in (A) showing that the tissue consists of inflammatory cells. (C) A magnified view of the black box in (B) demonstrating that these cells appear to be primarily mononuclear in nature, likely representing macrophages or fibroblasts. (D) Magnified view of the seeded scaffold of the black box in (A), bottom image showing that the tissue is primarily inflammatory. (E) Additional representative H&E images of unseeded and seeded scaffolds at each time point (scale bars = 100 μm). In general, they all demonstrate the presence of inflammation with some sections containing capillaries from the omentum (for example, the 4-week unseeded scaffold). H&E, hematoxylin and eosin. Color images are available online.