Fig. 2.

Iron status modulates microbiota diversity. Weighted UniFrac Principal Coordinate Analysis (PCoA) plot of a representative experiment of cohorts of mice a fed an iron-deficient (Def), iron-replete (Rep) or iron-supplemented (Sup) diet; b fed an iron-deficient diet and either infused with intravenous iron (Def+IV iron) or chronically transfused (Def+Trans); c fed an iron-replete diet and either infused with intravenous iron (Rep+IV iron) or chronically transfused (Rep+Trans); and d fed an iron-supplemented diet and either infused with intravenous iron (Sup+IV iron) or chronically transfused (Sup+Trans). e Shannon alpha diversity index of the mice represented in a–d and f Spearman correlation dot plot comparing logarithm of Shannon alpha diversity index and liver iron for each mouse in a representative experiment; n = 5 mice per group; *p < 0.05, **p < 0.01