Table 1.

Mixed model analysis of relative abundance of bacterial taxa

		Fixed effects
Taxonomic rank	Denomination	Diet		Treatment
		Def	Sup	IV iron	Transfusion
Phylum	Proteobacteria	3.91**	−2.25	−2.41*	−2.98*
Class	Bacilli	5.97***	−4.54*	−6.98***	−3.84*
	Clostridia	−8.16***	2.54	10.73****	9.19****
	Betaproteobacteria	2.63***	−0.9	−0.36	−0.88
Order	Lactobacillales	6.02***	−4.65*	−7.24****	−4.18*
	Clostridiales	−7.72***	2.97	10.46****	9.03****
	Burkholderiales	2.63***	−0.9	−0.36	−0.88
Family	Lachnospiraceae	−4.12****	0.67	0.51	0.22
	Streptococcaceae	0.26**	−0.23*	−0.36****	−0.19*
	Peptococcaceae	−1.41	2.45**	3.45****	2.25**
	Clostridiaceae	−1.77**	2.39**	2.81****	2.44***
	Lactobacillaceae	5.76***	−4.42*	−6.89***	−3.99*
Genus	Parabacteroides	4.56****	−1.80*	−3.76****	−2.96**
	Ruminococcus	−0.14	1.32****	0.14	0.04
	Lactobacillus	5.76***	−4.42*	−6.89***	−3.99*
	Dehalobacterium	−0.04	0.12	0.30***	0.34****
Species	Bacteroides acidifaciens	−3.17****	−1.18	0.04	1.46*
	Parabacteroides distasonis	2.14**	−1.07	−1.29*	−1.14

Parameter estimates of taxa modulated by dietary iron, intravenous (IV) iron, or chronic transfusion with a p value < 0.0001 for the test of fixed effects. Positive and negative values indicate increasing and decreasing trend, respectively, when compared with cohorts of mice fed an iron-replete diet alone. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001