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. 2019 Sep 24;6:179. doi: 10.1038/s41597-019-0185-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — RNA integrity of samples. Following denervation for the designated durations, denervated and contralateral intact gastrocnemii were harvested and homogenized directly in TRIzol, and total RNA was column-purified. RNA samples were reverse-transcribed to cDNA and sequenced on an Illumina platform. Representative RIN tracings from one biological replicate of the cohort, showing total RNA isolated from intact gastrocnemii (a) and paired contralateral denervated gastrocnemii (b). RNA isolated from denervated and intact muscle showed similar quality (c).