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. 2019 Sep 24;6:179. doi: 10.1038/s41597-019-0185-4

Table 8.

Day 30 post-denervation alignments.

CTL-30-1 CTL-30-2 CTL-30-3 CTL-30-4 DN-30-1 DN-30-2 DN-30-3 DN-30-4
Number of input reads 52,742,878 46,463,403 57,501,219 60,468,553 73,590,727 48,399,322 51,665,579 54,577,655
Average input read length 138 138 138 138 138 138 138 138
Number of uniquely mapped reads 40,308,924 38,146,896 48,521,247 49,438,872 58,520,852 41,879,303 45,337,494 46,751,912
Uniquely mapped reads (%) 76.43 82.10 84.38 81.76 79.52 86.53 87.75 85.66
Average mapped length 137.26 137.46 137.68 137.65 137.6 137.5 137.65 137.55
Number of splices: Total 9,002,164 12,162,162 17,453,824 21,318,005 19,800,202 14,238,822 17,245,138 17,175,362
Number of splices: Annotated (sjdb) 8,779,541 11,999,102 17,264,634 21,090,387 19,528,567 14,056,750 17,044,059 16,948,110
Number of splices: GT/AG 8,902,807 12,063,473 17,319,770 21,157,409 19,623,217 14,122,315 17,105,745 17,027,539
Number of splices: GC/AG 50,013 69,863 105,703 125,956 132,947 88,561 112,478 111,959
Number of splices: AT/AC 4,129 5,439 7,989 9,587 11,133 6,876 8,957 9,017
Number of splices: Non-canonical 45,215 23,387 20,362 25,053 32,905 21,070 17,958 26,847
Mismatch rate per base (%) 0.41 0.34 0.18 0.18 0.21 0.23 0.16 0.22
Deletion rate per base 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00
Deletion average length 1.35 1.54 1.52 1.54 1.59 2.1 1.61 1.85
Insertion rate per base 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.01
Multi-Mapping Reads:
Number of reads mapped to multiple loci 7,401,284 5,478,248 6,190,600 8,046,293 8,580,950 4,181,432 4,632,180 5,281,885
% of reads mapped to multiple loci 14.03 11.79 10.77 13.31 11.66 8.64 8.97 9.68
Number of reads mapped to too many loci 1,771,867 704,627 353,874 396,886 672,558 312,239 158,758 369,051
Unmapped Reads:
% of reads unmapped: too many mismatches 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
% of reads unmapped: too short 4.84 3.82 3.70 3.98 7.27 3.68 2.63 3.59