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. 2019 Sep 24;9:13748. doi: 10.1038/s41598-019-50141-z

Figure 2.

Figure 2

Differential effect of increasing iodoacetate concentration on the viability and apoptotic response of hormone-responsive MCF7 and triple-negative MDA-MB-231 breast cancer cell lines. (A,B) IA-induced cytotoxicity of MCF7 and MDA-MB-231 was assessed using WST-1 after incubation for 4 h (A) or 24 h (B). (C) Representative flowcytometric scatter plots of AnnexinV/PI-based detection of early and late apoptosis in MCF7 and MDA-MB-231 following treatments with 0 or 15 µM IA for 4 or 24 h. (D–G) Quantification of 4 subpopulation AV−/PI−, AV−/PI+, AV+/PI−, AV+/PI+ in the two cell lines after 4 or 24 h IA treatments. (H–J) mRNA Relative fold expression changes for both MCF7 and MDA-MB-231 BAX (H), BAK (I), and Caspase 9 (J). Quantification of apoptotic subpopulations was carried out over 3 independent runs where (*) indicates p < 0.05, **p ≤ 0.01, ***p ≤ 0.001.