Fig. 1.

Green fluorescent protein incorporation into the genome of hiPSC. a Green GFP-positive colonies after nucleofection. b Confirmation of correct GFP insertion with the polymerase chain reaction and southern blotting. c–e Colonies of green GFP-positive B10 clones selected for differentiation (c). A detail of the B10 GFP + colony (d). A bright-field image of B10 GFP + colony (e). f–n show stable and persistent GFP insertion throughout cell differentiation, including bright field images, in the DE (f–h), AFE (i–k), and VBP before seeding on the collagen constructs (l–n). Scale bars in the panels of a, c, e, f, h, i, k, l, n = 400 μm. Scale bars in the panels of d, g, j, and m = 200 μm. AFE anterior foregut endoderm, DE definitive endoderm, GFP green fluorescent protein, hiPSC human induced pluripotent stem cells, VBP vocal fold basal progenitors. Source data provided as a Source Data file