Table 1.
Primers used for PCR and sequencing (preferred primers in bold), with information provided on respective gene fragment, primer name, direction (forward, F or reverse, R), primer sequence, standard PCR annealing temperature, utilization (PCR/ sequencing), and reference. Primer annealing temperatures used for sequencing at Macrogen were usually 50 °C (47–50 °C).
| Gene region | Primer name | F/R | Primer sequence 5'–3' | PCR annealing temperature (°C) | PCR/ Sequencing | Reference |
|---|---|---|---|---|---|---|
| COI | SymF1 | F | TTTCAACWAATCATAAARAYATTGG | 49 | PCR, seq | (Prous et al. 2016) |
| COI | SymF4 | F | AAATGATTATTYTCWACWAATCAYAA | 50 | PCR, seq | This study |
| COI | sym-C1-J1718 | F | GGAGGATTTGGAAAYTGAYTAGTWCC | 49 | PCR, seq | (Nyman et al. 2006) |
| COI | symC1-J1751 | F | GGAGCNCCTGATATAGCWTTYCC | 47 | seq | (Prous et al. 2016) |
| COI | SymR1 | R | TAAACTTCWGGRTGICCAAARAATC | 47 | PCR, seq | (Prous et al. 2016) |
| COI | SymR2 | R | TAAACTTCTGGRTGTCCAAARAATCA | 47 | PCR, seq | (Prous et al. 2016) |
| COI | A2590 | R | GCTCCTATTGATARWACATARTGRAAATG | 49 | PCR, seq | (Normark et al. 1999) |
| NaK | NaK_263F | F | CTYAGCCAYGCRAARGCRAARGA | 59 | PCR, seq | (Prous et al. 2017) |
| NaK | NaK_809F | F | GCWTTYTTCTCNACSAAYGCSGTNGARGG | 55 | PCR, seq | (Prous et al. 2017) |
| NaK | NaK_907Ri | R | TGRATRAARTGRTGRATYTCYTTIGC | 54 | PCR, seq | (Prous et al. 2017) |
| NaK | NaK_910R | R | TGRATRAARTGRTGRATYTCYTT | 50 | PCR, seq | (Prous et al. 2017) |
| NaK | NaK_1250Fi | F | ATGTGGTTYGAYAAYCARATYATIGA | 56 | PCR, seq | (Prous et al. 2017) |
| NaK | NaK_1250Fv2 | F | ATGTGGTTYGAYAAYCARATHATIGA | 56 | PCR, seq | This study |
| NaK | NaKRev475 | R | TCGATRATYTGRTTRTCRAACCACAT | 56 | seq | (Leppänen et al. 2012) |
| NaK | NaK_1498R | R | ACYTGRTAYTTGTTNGTNGARTTRAA | 52 | PCR, seq | (Prous et al. 2019) |
| NaK | NaK_1918R | R | GATTTGGCAATNGCTTTGGCAGTDAT | 59 | PCR, seq | (Prous et al. 2017) |
| POL2 | POL2_104Fi | F | GYATGTCAGTYACNGATGGIGG | 59 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_104Fv2 | F | CGNATGTCNGTNACNGAYGGIGG | 60 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_574R | R | TCYTCRTTNACRTGYTTCCAYTCNGC | 59 | seq | (Prous et al. 2019) |
| POL2 | POL2_599F | F | GARTGGAARCAYGTVAAYGARGA | 54 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_797F | F | ATGTAYGGNTCNGCNAARAAYCARGA | 58 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_889R | R | TGRAAYTGYARCATYTTWATRTTYTC | 52 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_928R | R | GGCATNCCNGGCATRTCRTTRTCNAC | 59 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_1388F | F | CAYAARATGAGTATGATGGG | 51 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_1459R | R | TTCATYTCRTCNCCRTCRAARTC | 52 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_1706F | F | TGGGAYGGNAARATGCCNCARCC | 60 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_1732R | R | GARAADATYTGYTTNCCNGTCCA | 55 | PCR, seq | This study |
| POL2 | POL2_1759R | R | ATCATRTTNACRTTNCCNGGDATDAT | 55 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_1777Ri | R | GTRCTGTGIGTYCKDATCATRTT | 55 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2 hym 3F | F | ACNCACAGYACNCAYCCN GAYGA |
56 | seq | (Malm and Nyman 2015) |
| POL2 | POL2_2423F | F | CATTTYATHAARGAYGAYTAYGG | 51 | seq | (Prous et al. 2019) |
| POL2 | POL2_2509R | R | TTNACRGCRGTATCRATNAGACCYTC | 60 | PCR, seq | (Prous et al. 2019) |
| POL2 | POL2_2569R | R | TGNACCATNACNGAYTCCATAGCYTTDAT | 60 | PCR, seq | This study |
| POL2 | POL2_2725R | R | GGATCRAAYTTRAAYTTYTTYTC | 50 | PCR, seq | (Prous et al. 2019) |
| TPI | TPI_29Fi | F | GYAAATTYTTYGTTGGNGGIAA | 52 | PCR, seq | (Prous et al. 2016) |
| TPI | TPI385Fi | F | GTRATYGCNTGYATYGGIGARA | 52 | seq | (Prous et al. 2016) |
| TPI | TPI 275Ri | R | GCCCANACNGGYTCRTAIGC | 56 | seq | (Malm and Nyman 2015) |
| TPI | TPI706R | R | ACNATYTGTACRAARTCWGGYTT | 52 | PCR, seq | (Prous et al. 2016) |
| TRRAP | TRRAP_833F | F | AAYAARGARGTNTTYGTNGAYTTYATGGG | 58 | PCR, seq | This study |
| TRRAP | TRRAP_1658F | F | CARTCNAARCARTTYCARCCNAARGARAC | 60 | seq | This study |
| TRRAP | TRRAP_1702R | R | GGNGGNCCDATNGTRTARATRTC | 56 | seq | This study |
| TRRAP | TRRAP_1831R | R | AADATYTCYTGRAANGTYTGNGGRTTCAT | 59 | seq | This study |
| TRRAP | TRRAP_2648Fi | F | ATGATGATHGARCCNCARAARYTNGAITA | 58 | PCR, seq | This study |
| TRRAP | TRRAP_3046R | R | TGNGCDATNGCNACCATNGTRTARTG | 60 | PCR, seq | This study |
| TRRAP | TRRAP_3482Fi | F | GTNTCNAAYGGNGCHATHGAYATGGCIAA | 62 | seq | This study |
| TRRAP | TRRAP_3685Ri | R | ACYTCYTTRTGNGGYTCCATNACYTCIGT | 62 | PCR, seq | This study |
| TRRAP | TRRAP_4086F | F | CARGARGCNGCNTTYGARTGYATG | 59 | seq | This study |
| TRRAP | TRRAP_4213Ri | R | CTRAANGTRCTNGGRAANARYTGIGT | 56 | PCR, seq | This study |