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. 2019 Sep 24;9:13800. doi: 10.1038/s41598-019-49953-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NER protein expression in HUVEC, HUASMC and HPC not treated and treated with BPDE. (a) Dose-dependent expression of NER core proteins was analyzed by immuno-detection in total cell extracts 24 h after exposure to 0, 0.5 and 1 µM BPDE. HSP90 was used as loading control. HUVEC, HUASMC and HPC extracts were loaded onto the same blot in each experiment in order to have a direct comparison. The same extracts were used in different immunoblots for the indicated proteins. The loading control (HSP90) shown in the figure is a representative example. The numbers below each blot for the corresponding repair protein indicate the relative expression level, in which HUVEC control was set to 1. Quantitative analysis of blot intensity was done with ImageJ and related to the HSP90 control in the same blot. Expression data of a representative experiment are shown. (b) mRNA expression of DDB2 and XPC in HUVEC, HUASMC and HPC following treatment with 0, 0.5 and 1 µM BPDE. Cells were harvested 24 h after treatment and the expression was determined by RT-PCR. Data show the mean ± SD of three independent experiments. The relative mRNA expression level of the non-treated control was set to 1.0. The difference to the control was not significant (ns) or significant (p = 0.05 *0.01**0.001***).