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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Anal Biochem. 2019 Jun 13;584:113328. doi: 10.1016/j.ab.2019.05.017

Fig. 1. Peptide epitope-linked β2-microglobulin HLA-A2 constructs.

Fig. 1.

(A) Schematic of peptide exchange using epitope-linked β2-microglobulin. (B) Schematic of constructs used to express ELBM (SS: secretion signal sequence; bZIP: basic leucine zipper; BSP: BSP85 peptide; H: His6 tag; P: peptide: T: thrombin cleavage site; aZIP: acidic leucine zipper). Linker sequences shown in green. Vertical line in thrombin sequence shows expected cleavage site. The peptide epitope sequence shown is from the ML9 peptide, while in some experiments other sequences were used (see below). Not drawn to scale. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)