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. 2019 May 14;70(18):4919–4930. doi: 10.1093/jxb/erz230

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© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 3. — Functional characterization of AtAMT1;3 T494 phospho-mutants in Xenopus oocytes. Kinetic properties of ammonium-induced currents in oocytes injected with AtAMT1;3, and AtAMT1;3 phospho-mutants T494A and T494D. Inward currents were plotted under conditions of 30~1000 μM NH₄Cl at −100 mV. Bars indicate means ±SD (n≥4). From a fit with the Michaelis–Menten equation, V_max and K_m were calculated.