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. 2019 May 14;70(18):4903–4918. doi: 10.1093/jxb/erz217

Table 1.

List of oligonucleotides used in this study

Code 5′–3′ sequence Use Reference
HsfA4A-qF CTTTGAACCTATCGCCGTGT HSFA4A (AT4G18880) qRT-PCR Pérez-Salamó et al., 2014
HsfA4A-qR TGTGTGTGAAGAAGGGCTTG HSFA4A (AT4G18880) qRT-PCR Pérez-Salamó et al., 2014
ZAT12-qF GACGCTTTGTCGTCTGGATT Zat12 (AT5G59820) qRT-PCR Pérez-Salamó et al., 2014
ZAT12-qR GTGTCCTCCCAAAGCTTGTC Zat12 (AT5G59820) qRT-PCR Pérez-Salamó et al., 2014
WRKY30-qF AGAGCGATGATTCCGATCAAG WRKY30 (AT5G24110) qRT-PCR Besseau et al., 2012
WRKY30-qR CATCGTCCAGCGTTCTATCAA WRKY30 (AT5G24110) qRT-PCR Besseau et al., 2012
Hsp17.6A-qF CCAAAGAAAAAGCCAAGAAGC Hsp17.6A (AT5G12030) qRT-PCR Pérez-Salamó et al., 2014
Hsp17.6A-qR TGGAAACCTTCCAAACTCCA Hsp17.6A (AT5G12030) qRT-PCR Pérez-Salamó et al., 2014
ER8A GCTTGGGCTGCAGGTCGAGGCTAA Amplification of inserts in pER8 Papdi et al., 2008
ER8B CTGGTGTGTGGGCAATGAAACTGATGC Amplification of inserts in pER8 Papdi et al., 2008
GAPDH2_qF AATGGAAAATTGACCGGAATGT GAPDH2 (AT1G13440) qRT-PCR Papdi et al., 2008
GAPDH2_qR CGGTGAGATCAACAACTGAGACA GAPDH2 (AT1G13440) qRT-PCR Papdi et al., 2008
ZAT12-chipF GTTGTCATGCCTTTGCTGG Zat12 (AT5G59820) ChIP qPCR This study
ZAT12-chipR GGTCAGGTGTATTCTAGAAAGTGG Zat12 (AT5G59820) ChIP qPCR This study
WRKY30-chipF AAAGGATCGAGAAGCAGAGAAC WRKY30 (AT5G24110) ChIP qPCR This study
WRKY30-chipR CGAAGAAAGCTGATGTGGTTTG WRKY30 (AT5G24110) ChIP qPCR This study
Hsp17.6A-chipF ACTTCTCCAACGATCAAGACG Hsp17.6A (AT5G12030) ChIP qPCR This study
Hsp17.6A-chipR AGCAATGTAGAGAGAGCGATTG Hsp17.6A (AT5G12030) ChIP qPCR This study
TUA3-chipF TTTCCTAAGCGGTTTCAGATTAAAG TUA3 (AT5G19770) ChIP qPCR This study
TUA3-chipR GAATTGCTACTTAAGCTATCTTCCTAAC TUA3 (AT5G19770) ChIP qPCR This study
T7 promoter TAATACGACTCACTATAGGG HSFA4A (AT4G18880) cloning This study
T7 terminator TATGCTAGTTATTGCTCAG HSFA4A (AT4G18880) cloning This study
HSFA4A-S309D CAAATCTCCTCCTGATCCAAGGATCATC HSFA4A (AT4G18880) mutagenesis This study
M13 F GTAAAACGACGGCCAGTG pBluescript II SK+, sequencing
M13 R GGAAACAGCTATGACCATG pBluescript II SK+, sequencing
HSFA4A-HindIII TGAGCAAGCTTATGGATGAGAATAATCATGGAGTTTC BiFC vector cloning Pérez-Salamó et al., 2014
HSFA4A-SmaI-NoStop TGATTCCCGGGACTTCTCTCTGAAGAAGTCAGAT BiFC vector cloning Pérez-Salamó et al., 2014