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. 2019 Jun 6;70(18):4763–4774. doi: 10.1093/jxb/erz270

Fig. 2.

Fig. 2.

CEP3 levels affect root meristem size and cell cycle progression. (A, C) Confocal images of propidium iodide-stained 6-day-old root meristems. (B, D) Enumerated MZ cell number (n≥14). Red and blue arrowheads in (A) and (C) indicate the MZ and TZ boundary, respectively. (A, B) Plants were grown on standard 1/2 MS medium. (C, D) Plants were grown on modified N-free 1/2 MS medium. (E–G) Confocal images of CEP3 peptide-treated and non-treated reporter lines grown on standard 1/2 MS medium without and with 1 µM CEP3 peptide: (E) pSCR2.0:SCR:GFP; (F) pWOX5:YFP, and (G) pCYCB1;1:CYCB1;1:GFP. (H) Number of pCYCB1;1:CYCB1;1:GFP-positive cells per primary root tip. Focal planes right through the root were examined in (H). n=30 root tips. *P<0.05; **P<0.01; ***P<0.001 (two-sample t-test, Genstat). Scale bars=10 μm in (A), (C), (E–G).