Table 2.

Sequences of the Primers Used for miRNA Analysis by Quantitative Real-Time PCR

qPCR Forward Primer		Specific RT Primer
cel-miR‒39-5p.SpFwd2	GCGCTCACCGGGTGTAAATC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAGCT
hsa-miR‒122-5p.SpFwd2	GCGCTGGAGTGTGACAATGG	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAACA
hsa-miR‒132-3p.SpFwd2	CGCTGTTAACAGTCTACAGCCA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGACCA
hsa-miR‒1323.SpFwd2	GCGTCAAAACTGAGGGGCA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGAAAA
hsa-miR‒136-5p.SpFwd2	TGGTCGGACTCCATTTGTTTTGAT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCCATC
hsa-miR‒182-3p.SpFwd2	GCTGTCGTGGTTCTAGACTTGC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGTTG
hsa-miR‒210-3p.SpFwd2	GCGCTGTGCGTGTGACAGC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGCC
hsa-miR‒29a-3p.SpFwd2	GCTGTCGTAGCACCATCTGAAAT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCG
hsa-miR‒29b-3p.SpFwd2	GCTGTCGTAGCACCATTTGAAATC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACACT
hsa-miR‒342-3p.SpFwd2	GCGCTCTCACACAGAAATCGC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACGGGT
hsa-miR‒376c-5p.SpFwd2	GCTGTCGGGTGGATATTCCTTC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACATA
hsa-miR‒483-3p.SpFwd2	GCGCTCACTCCTCTCCTCC	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGACG
hsa-miR‒494-3p.SpFwd2	GCGCTGAAACATACACGGGA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAGGTT
hsa-miR‒517-5p.SpFwd2	GCGCCCTCTAGATGGAAGCA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGACAG
hsa-miR‒517a-3p.SpFwd2	GCGCATCGTGCATCCCTTTA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACACTC
hsa-miR‒518b.SpFwd2	GCGCAAAGCGCTCCCCTTT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCTCT
hsa-miR‒520h.SpFwd2	GCGCACAAAGTGCTTCCCTT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCTA
hsa-miR‒525-5p.SpFwd2	GCGCTCCAGAGGGATGCA	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGAAAG
qPCR reverse primer	GTGCAGGGTCCGAGGT	Shared-mir_reva

^aPrimer that targets the added stem-loop sequence in reverse transcription.